... 3 h to determine the mechanisms. Results illustrated that CS activated the Gsα protein subunit, but not Giα, to induce ... steroidogenesis in MA-10 cells, strongly suggesting that CS had activated the PKC pathway. Co-treatment with PKA and PKC inhibitors ... 61% of CS-stimulated steroid production, indicating that CS simultaneously activated PKA and PKC pathways. Moreover, CS induced the expression of ...