Hyaluronidase
DESCRIPTIONHyaluronidase is a glycoprotein containing 5% mannose and 2.7% glucosamine. Optimum pH range is 4.5-6.0. The enzyme catalyzes the hydrolysis of endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfate A and C (but not B), primarily to tetrasaccharide residues.The Hyaluronidase is extracted from bovine testes, and has been chromatographically purified to remove trace contaminating proteases.
Specification
Item | Specification | Method |
Appearance | A white or yellow-white powder | |
Identification | Complies | EP |
Appearance of solution | Clear | EP |
pH | 4.5 - 7.5 | EP |
Loss on drying | Less than 5.0% | EP |
Bacterial endotoxins | Less than 0.2 IU per IU of hyaluronidase | EP |
Limit of tyrosine | Less than 0.25 µg per USP Hyaluronidase U. | USP |
Activity | More than 300 IU/mg powder | EP |
Hyaluronidase