1. Sample flowing rate: 4~450aal/minaa 2. Refractive index range: 1.30 -1.36 ;

Name:Optical SPR Bioanalyzer

Model: HPSPR-6000

I. Brief :

HPSPR-6000 Bioanalyzer use touching LCD , which can display SPR curve and sensor responding plot , it is convenient to observe and analyse for customers ; It create optical sensing system by Kretschmann structure , and take 850nm (wave length) LED as incident light source , Analyse and test sample through changing light incident angle ; Others, we can supply many kinds of sensor chips for surface carboxylation , customers can customize chips according to their demands ; Use internal setting recovery system to sample , recover tested sample to tubule . Control system is made by precise peristaltic pump with wide-range (0.006ml/min – 290ml/min) volume flow , it can meet different customers’ demands .

II. Testing Principle:

When detecting , First fixed layer film (Au) for biomolecular recognition on surface of sensor chip , testing sample flows through surface of chip , If molecular in sample solution can interact with biomolecular recognition film , it can cause index of refraction change on surface of Au film , and cause SPR angle change . People can get information of analyte’s concentration, affinity, kinetic constants and specificity through detecting SPR angle change .

III. Technical parameter

1. Sample flowing rate: 4~450μl/min;

2. Refractive index range: 1.30 -1.36 ;

3. Single channel area & volume in flowing cell: 3.6mm² , 2.5ul .

4. Stationary wave length: 850nm ;

5. Ka (association rate constant): 103~107M-1s-1

6. Kd (dissociation rate constant): 10-6~10–1s-1

7. Power: 220±15% V AC,50 Hz ;

8. Concentration range: <10-10~10-3M;

9. KD (kd / ka) : 10-4~10-10M ;

10. Displaying system: Touching screen, don’t need computer;

11. Sensing system: Controlled by microprocessor;

12. Detecting dynamic range: 0 - 999999RU.

IV. Instrument Performance:

1. System performance: it’s fit for detecting dynamic response to many kinds of biomolecules ;

2. Biosensor chip: single sensitive biological membrane can be resuable ;

3. Way for sample into the channel : Manual / by hand (peristaltic pump) ;

4. Three channels and single chanel for smaple injection way;

5. Internal / External setting injection pump for optional samples ;

6. Minimum testing limit (take clenbuterol as example) : 1.0 μg/L ;

7. Reproducibility RSD (take ethanol solution as example) : 9.23×10-3 .

VI. Applications:

 Life sciences ; Food safety ; Environmental Detection ; Biomedical ; Rapid detection for toxins and antibiotics ; Proteomics ; Drug screening and actual detection for pharmacokinetics ; Biomolecules special peptides and coupled molecules detection ; Viruses and pathogentic molecular / Research for protein and receptor ; Molecular recognition ; Immunoassay, etc . It’s specially suitable to use in Colleague, University and Research Institutes for scientific research and teaching experiments .

Optical SPR Bioanalyzer